Kinetic mechanism ofl-α-glycerophosphate oxidase fromMycoplasma pneumoniae

Structure and proposed mechanism of L-α-glycerophosphate oxidase from Mycoplasma pneumoniae.

UNLABELLED The formation of H2 O2 by the FAD-dependent L-α-glycerophosphate oxidase (GlpO) is important for the pathogenesis of Streptococcus pneumoniae and Mycoplasma pneumoniae. The structurally known GlpO from Streptococcus sp. (SspGlpO) is similar to the pneumococcal protein (SpGlpO) and provides a guide for drug design against that target. However, M. pneumoniae GlpO (MpGlpO), having < 20%...

Investigation of the Catalytic Mechanism of Mycoplasma Pneumoniae L-alpha-glycerophosphate Oxidase: Mutation of the Proposed Catalytic Base

Lactic and Α-glycerophosphate Dehydrogenases in Insects

Different tissues, especially muscles, from insects belonging to various groups were extracted and studied for their lactic dehydrogenase (LDH) and alpha-glycerophosphate dehydrogenase (GDH I) activities from the comparative point of view. In all cases assays of flight muscle extracts showed extremely low values of LDH activity whereas the GDH activities were surprisingly high. The activities i...

The Α-glycerophosphate Cycle in Drosophila Melanogaster

"Null" mutations previously isolated at the alphaGpdh-1 locus of Drosophila melanogaster, because of disruption of the energy-producing alpha-glycerophosphate cycle, severely restrict the flight ability and relative viability of affected individuals. Two "null" alleles, alphaGpdh-1(BO-1-4), and alphaGpdh-1(BO-1-5,) when made hemizygous with a deficiency of the alphaGpdh-1 locus, Df(2L)GdhA, wer...

Kinetic mechanism of pyranose 2-oxidase from trametes multicolor.

Pyranose 2-oxidase (P2O) from Trametes multicolor is a flavoprotein oxidase that catalyzes the oxidation of aldopyranoses by molecular oxygen to yield the corresponding 2-keto-aldoses and hydrogen peroxide. P2O is the first enzyme in the class of flavoprotein oxidases, for which a C4a-hydroperoxy-flavin adenine dinucleotide (FAD) intermediate has been detected during the oxidative half-reaction...